Re: [Histonet] Alzheimer stain

From:Rene J Buesa

I use the following procedure for Bielschowsky:
  1- silver solution (20% aq.)
  2- 0.39% aq. formaldehyde (developer)
  3- 0.28% aq. ammonium hydroxide (interrupter)
  4- 5% aq. sodium thiosulfate (reducing solution).
  I never used sodium carbonate
  René J.

Behnaz Sohrab  wrote:
  Question regarding Sodium Carbonate Solution in Bielschowsky method for Alzheimer. Can I substitute Carbonate? Or any one has a better method for this stain? ( I do not have Sodium Carbonate, and no time to order it).

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