RE: [Histonet] embedding problem small zebrafish

From:"Dickey, Coral"


Check out a paper in ScienceDirect available online at Entitled: High-throughput zebrafish histology by Sabaliauskas et. Al.

Good Luck!

Coral Dickey
Histology Specialist I

Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City,  Missouri  64110
Phone:  816-926-4305

-----Original Message-----
From: [] On Behalf Of Andrea Hooper
Sent: Tuesday, March 25, 2008 10:16 AM
Subject: Re: [Histonet] embedding problem small zebrafish

Have you checked out the histological methods section of "The Zebrafish Book" by Monte Westerfield, on available online here:
I don't think it has paraffin embedding protocols but perhaps there are some other hints will help? In any case, it's an amazing resource.

I am also wondering if it might help to embed in agarose before paraffin embedding - especially those 44 hour old critters who are tiny tiny tiny. Unfortunately most of my experience in that regard lies with frozen sectioning so I don't have too much to add.

----- Original Message -----
From: Barbara Verstraeten 
Date: Tuesday, March 25, 2008 3:50 am
Subject: [Histonet] embedding problem small zebrafish

> Hello,
> I work on zebrafish from 44 hours to 6 days old (so they are very
> small; max. 0.5 cm). I would like to perform immostainings on them
> (such as E-cadherin staining). I've embedded my species in technovit
> 9100 but the cutting
> of such small animals is very difficult.
> I did some stainings already and the antibody works but the problem
> is
> that my tissue is of very bad quality!! When I have 50 sections
> only 1
> is usefull.
> This is not very good!
> Can someone give me any suggestions on how to embed them so that they
> are more easy to section and that I can still stain them with
> different antibodies?
> Thank you all in advance!
> Kind regards,
> Barbara Verstraeten
> University of Ghent
> Belgium
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