Um no, Joe.....
If you do that then the xylene/ wax impregnated tissue won't mix with the
formalin (as you say), anyway it's fixed that's not the issue, so why waste
time fixing again?. Melt the blocks down, as you say, then pass through
xylem until they are transparent, well as transparent as the poor processing
allows. You then place into 100% alcohol until properly dehydrated denoted
by the xylene making them transparent (trial and error I'm afraid as you
don't know how dehydrated they are). When clear impregnate with wax as
before; you may note that the tissue becomes more brittle because of
increased length of time in reagents and especially wax. But less so than
Joe says as you are not doubling the processing time which must impact
adversely on ICC.
Have fun.
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito
Sent: 24 March 2008 00:46
To: Lynn Wade; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Reprocessing tissue
Lynn,
ThermoFisher Scientific and Ventana Medical Systems have systems that you
can barcode the paperwork, which then makes bar-coded blocks which then
makes bar-coded slides. In each step, the barcodes are read to ensure that
the correct specimen is being processed. If there is a mis-match, an alarm
beeps alerting the user.
For reprocessing tissue, we just melt the blocks down, place the tissue back
in the block and put the blocks in formalin to be processed with new cases.
Whatever area did not process the first time will take up the formalin, and
graded alcohols. When the tissues reach xylene, the paraffin is dissolved
and everything get infiltrated. The areas that have been processed will
repel the formalin and alcohols until they are immersed into xylene. I find
this method is a lot easier on the tissues, especially if IHC is performed
on them later.
As far a an electronically created list of blocks going into a processor, I
haven't heard of any.
Hope this helps.
Joe
----- Original Message -----
From: "Lynn Wade"
To:
Sent: Sunday, March 23, 2008 6:31 PM
Subject: [Histonet] Reprocessing tissue
Hi folks:
I am wondering if anyone has had an incident in Patholgy lab where on the
tissue processor the reagents got switched inadvertantly. For instance, the
80% alcohol was inadvertantly placed in the last 100% alcohol slot and thus
water was reintroduced into the tissue just before xylene, clearing amd
paraffin.
Has anyone had this occur and how did you recover the tissue?
Also, can anyone tell me if there is such a processor that has a system that
can be used to log in the cassette numbers that are put onto the processor
so that in the event of some incident such as we had the retrieval of the
exact specimens can be done electronically?
And lastly can anyone tell me if they have a fully barcoded system whereby
path specimens arrive barcoded and every document, slide and block has a
barcode that allows for tracking of the tissue at all times?
We are looking at processes and trying to close some gaps.
Lynn Wade
Program Manager, Safety & Quality Management
Medical Services & Diagnostics
Eastern Health
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