RE: [Histonet] Choosing an antibody

From:"Liz Chlipala"


F4/80 does not work on rat, it's a marker for activated macrophages in
mouse tisse.  For rat you want the clone ED-1.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 682-3949
fax (303) 682-9060
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive
Unit E
Longmont, CO 80504

-----Original Message-----
[] On Behalf Of Till,
Sent: Monday, April 14, 2008 11:55 AM
Subject: [Histonet] Choosing an antibody

I am searching for an F4/80 antibody to used on FFPE rat livers. I have
found two possibilities (though if anyone happens to have an antibody
suggestion please let me know) and I am wondering which would be better,
or if it makes much difference. They are both from the same company but
the sources differ.
One is a rabbit polyclonal raised against amino acids w/in and
extracellular domain, and the other is raised against a peptide mapping
at the C-terminus.
I have encountered this before and always wondered what is the
difference and if one is better. Thanks.


Renee' Till, HT (ASCP)

Research Assistant

Arkansas Children's Nutrition Center


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