What kind of hematoxylin? I thought this would be impossible since we no longer use mercury! Joyce -----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun LangSent: Tuesday, April 01, 2008 12:57 PMTo: 'Marshall Terry Dr,Consultant Histopathologist'Cc: histonet@lists.utsouthwestern.eduSubject: AW: [Histonet] Bottomless cup of coffeeIf it works, ... it's acceptable. Or have you noticed any problems in staining, that can be caused by this?And perhaps there are standardizised times and amounts for filling up ;).I also remember something similiar in our lab before automated staining. And it was a kind of religion, that the haemalaun (Mayers) even would turn better with time in the pot. We always had beautiful HEs. (And now we havealso.)Gudrun Lang Biomed. AnalytikerinHistolaborAkh LinzKrankenhausstr. 94020 Linz+43(0)732/7806-6754-----Ursprüngliche Nachricht-----Von: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Marshall Terry Dr,Consultant HistopathologistGesendet: Dienstag, 01. April 2008 17:57An: histonet@lists.utsouthwestern.eduBetreff: [Histonet] Bottomless cup of coffee I first came across this concept in Ottawa in 1962 - a cup of coffee that was replenished at no extra charge.In Oman in 1989, I came across the Indian vegetarian thali that was constantly replenished. (Pearl restaurant in Ruwi - highly recommended).Now I have come across the bottomless pot of haematoxylin, which has been in use in this lab since I came 8 years ago.Apparently it is just topped up now and then, and rarely discarded for fresh haematoxylin.Is this normal/standard/acceptable.I am shocked to the core!Terry_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonetConfidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc.