RE: AW: [Histonet] Kidney Bx question

From:"kemlo"



Terry it doesn't translate!!!

Men and women, Brits and Yanks, different species.



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall
Terry Dr,Consultant Histopathologist
Sent: 20 March 2008 14:32
To: Godfrey Guerzon; histoinfo@comcast.net; Gudrun Lang
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: AW: [Histonet] Kidney Bx question

Godfrey,
Nobody has said anything snide, or put anybody down.
Your post is typical of those lacking a sense of humour, and who think being
professional is synonymous with being formal.
It is not.

Terry 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Godfrey
Guerzon
Sent: 20 March 2008 14:17
To: histoinfo@comcast.net; Gudrun Lang
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: AW: [Histonet] Kidney Bx question

We are supposed to be professionals.  What we post are real issues in our
situation and ask for help.  Professionals don't make snide remarks and put
down our colleagues.  If we think that it will raise our stature as
professionals when we make a joke and condescending comments on things that
we need help on, we are greatly mistaken. A wise man once said "if you have
nothing good to say about anything - shut up".  We need to understand that
each situation is different - who are we to judge?  We post a real issue in
our own situation and we don't need "smart" colleagues make a joke of our
situation. Do we want to be treated as professionals - then let us
communicate as professionals.

No snide remarks or condescending comments - PLEASE!!! - Help and good ideas
are welcome - if you have nothiing to contribute to the issue at hand -
please don't waste your (and ours) valuable time with useless comments.

Godfrey

>>> "Gudrun Lang"  3/20/2008 9:53 AM >>>
Would you be so kind and explain, why you have to do a short run. Are these
indeed NTX-biopsies, that need a report as fast as possible?
Or is it just the fear to over-process the biopsies?

We have ntx from time to time, but even then, the clinicians wait for the
report until the next day, noon. So we can process the biopsies with our
regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG =3D
kind of trichrome), immunohistochemistry and immunofluorescence. 
We process the needlebiopsies in sponges and with the regular 13-hour
protocol. Regarding IHC a fixation-time under 6 hours could also drive to
problems.

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Ursprüngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von
histoinfo@comcast.net
Gesendet: Donnerstag, 20. März 2008 14:01
An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist;
histonet@lists.utsouthwestern.edu
Betreff: RE: [Histonet] Kidney Bx question


Rene,
Yes they are agitated. And the tearing problem that I originally described
is fairly infrequent. It's just that when it does happen we can't figure out
why. When it does happen nothing that we can see is any different than any
other day. Nothing in common as far as collection site either. I am
beginning to think more and more that it may be on days that we have 5 or 6
bxs that are all run together. But even then it is not every bx. I think we
will take better steps to prevent solution carryover and see what happens.
Of course it is so infrequent that we have this problem that it will be
months before we can say that we "fixed" the problem. I would still like to
compare times with others out there though. So please share your times for
hand processing.
Thanks,
Jennifer



-------------- Original message --------------
From: Rene J Buesa  

For hand processing at room temperature that schedule is too short, unless
the samples are agitated.
René J.

"Marshall Terry Dr, Consultant Histopathologist"
 wrote:
Do you have a train to catch?

Terry 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
histoinfo@comcast.net
Sent: 19 March 2008 17:14
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Kidney Bx question

We have been having some trouble with the inner surface of the tubules
looking torn look to the tissue. We hand process all our kidney bx's with
the times as follows.

1-formalin 15 minutes
2-50% ethanol 15 minutes
3-70% ethanol 15 minutes
4-80% ethanol 15 minutes
5-95% ethanol 15 minutes
6-100% ethanol 15 minutes
7-100% ethanol 15 minutes
9-xylene 15 minutes
10-xylene 15 minutes
Parraffin x2, 20 minutes each. 
They are then cut at 1 micron for PAS & JONES and 2 microns for H&E &
TRICHROME. 

We are trying to determine if this tearing is an artifact from processing,
collection or some other factor we haven't thought of. Has anyone else come
across this or a similar problem. All thoughts and suggestions welcome.

Jennifer Saunders HT (ASCP)
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