My boss has asked me about immunofluorescence staining in mouse
spleen: How do you get rid of fluorescent background in mouse
spleen? I suggested ammonia ethanol quenching which I know works in
bone marrow. Is there a better method? Also, my boss claims that
the macrophages have autofluorescence (formalin fixed paraffin
embedded tissue). I always thought the rbcs would be the
autofluorescent cells. Do the macrophages have autofluorescence?
Finally, is it reasonable to do frozen sections of mouse spleen? I
thought frozen sections fixed in ice cold acetone or acetone-methanol
might solve the problem, but I was told that frozen sections would be
much to thick to get any good immunofluorescent pictures on.
Thanks for any info and suggestions.
Histonet mailing list