[Histonet] collagen coating

From:"Hazel Stevens"


Hi All,


I have received excellent responses from Histonet in the past so thought I
would try this out on you all. 


I am coating tissue culture plates with collagen and trying to demonstrate
complete coverage. Other than an antibody to collagen I , is there a simple
stain I could apply that would demonstrate coverage without dissolving the
collagen. Collagen seems to dissolve in methanol which seems to be a
component of some of the basic protein stains. Should I fix before staining?

Thanks for your help. Much appreciated.



Hazel Stevens, 

Georgia Tech,

Atlanta, Georgia 30332-0405 U.S.A.


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