[Histonet] c. elegans

From:"Allen, Rhonda"



The only way I know to fix them without nicking them is to use a high pressure freezer and then use freeze substitution.


Thanks,

Rhonda

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Message: 4
Date: Wed, 19 Mar 2008 10:23:24 -0700
From: "Danielle Crippen" 
Subject: [Histonet] C. Elegans fixation
To: 
Cc: Cathy Vitelli 
Message-ID:
        
Content-Type: text/plain;       charset="us-ascii"

Hello Histo-experts,



Can you please share your favorite worm fixation methods with me??  I've
had great success in EM prep using 2% PFA 0.2% gluteraldehyde in
cocodylate buffer.  BUT-I had to "nick" the worms to get infiltration of
the fixative.  I now have a user who would prefer to avoid the "nicking"
technique for his application, but so far, we're experiencing
insufficient fixation.  Any/all advice is very welcome!!!



Many thanks in advance!



danielle



Danielle Crippen

Morphology and Imaging Core







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