The only way I know to fix them without nicking them is to use a high pressure freezer and then use freeze substitution.
Date: Wed, 19 Mar 2008 10:23:24 -0700
From: "Danielle Crippen"
Subject: [Histonet] C. Elegans fixation
Cc: Cathy Vitelli
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Can you please share your favorite worm fixation methods with me?? I've
had great success in EM prep using 2% PFA 0.2% gluteraldehyde in
cocodylate buffer. BUT-I had to "nick" the worms to get infiltration of
the fixative. I now have a user who would prefer to avoid the "nicking"
technique for his application, but so far, we're experiencing
insufficient fixation. Any/all advice is very welcome!!!
Many thanks in advance!
Morphology and Imaging Core
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