Use POPO3 Iodide, it is a nuclear membrane stain. 1:4000 concentration.
1uL Popo3 : 4000uL 1xPBS
>>> 04/09/08 12:59 PM >>>
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Today's Topics:
1. Re: Fading of slides (Rene J Buesa)
2. Nuclear Envelope/Membrane (Vernon Dailey)
3. Staining JB-4 sections (Aruna Somasiri)
4. Immunohistochemistry Machine (Ramona Turner)
5. Re: Immunohistochemistry Machine (William Stewart)
6. RE: SPAM-LOW: [Histonet] Immunohistochemistry Machine
(Douglas D Deltour)
7. RE: SPAM-LOW: [Histonet] Immunohistochemistry Machine
(Sebree Linda A.)
----------------------------------------------------------------------
Message: 1
Date: Tue, 8 Apr 2008 12:44:33 -0700 (PDT)
From: Rene J Buesa
Subject: Re: [Histonet] Fading of slides
To: "Webb, Dorothy L" ,
histonet@lists.utsouthwestern.edu
Message-ID: <114115.56489.qm@web65701.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Since it is something recently started, try to contact your water supply
treatment plant, they may have increased the chlorination level, and
that could be the culprit.
RenĪ J.
"Webb, Dorothy L" wrote:
---------------------------------
You rock. That's why Blockbuster's offering you one month of Blockbuster
Total Access, No Cost.
------------------------------
Message: 2
Date: Tue, 08 Apr 2008 17:32:26 -0400
From: Vernon Dailey
Subject: [Histonet] Nuclear Envelope/Membrane
To: histonet@lists.utsouthwestern.edu
Message-ID: <1bc8cc1c0fe3.1c0fe31bc8cc@imap.georgetown.edu>
Content-Type: text/plain; charset=us-ascii
Hi Histonet,
I need to know if there is a chemical stain specific to the nuclear
membrane....it could also stain the plasma membrane as long as good
nuclear membrane staining is present. any info would be appreciated. i
am aware of immuno appraches such as lamin B etc....chemical stain would
be best...thanks again.
Vernon Dailey
------------------------------
Message: 3
Date: Wed, 09 Apr 2008 08:23:27 -0700 (PDT)
From: Aruna Somasiri
Subject: [Histonet] Staining JB-4 sections
To: histonetlistsutsouthwesternedu
Message-ID: <64127.961207754607934.JavaMail.myubc2@handel.my.ubc.ca>
Content-Type: text/plain; charset=us-ascii
Hi Everyone,
I am having problems getting good Movats and Trichrom staining on JB-4
sections.
If some one have a good protocol to share much appreciated.
Thanks in advance for your help.
Regards
Aruna
------------------------------
Message: 4
Date: Wed, 9 Apr 2008 12:20:45 -0400
From: Ramona Turner
Subject: [Histonet] Immunohistochemistry Machine
To:
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
I am looking for a machine to process immunohistochemistry stains. I
want something small, that will do as much online as possible with
little hands on attention required. Any recommendations?
Ramona
Potomac Hospital
_________________________________________________________________
Going green? See the top 12 foods to eat organic.
http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51N1653A
------------------------------
Message: 5
Date: Wed, 9 Apr 2008 12:27:09 -0400
From: "William Stewart" Subject: Re: [Histonet] Immunohistochemistry Machine
To: "Ramona Turner"
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
<81a8916f0804090927g5c10f3c5y5b684d4e25147976@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
It is not small, but will do all steps of staining for MOST antibodies.
Try
Ventana Benchmark LT or XT.
Bill
On 4/9/08, Ramona Turner wrote:
>
>
>
>
> I am looking for a machine to process immunohistochemistry stains. I
want
> something small, that will do as much online as possible with little
hands
> on attention required. Any recommendations?
>
> Ramona
> Potomac Hospital
> _________________________________________________________________
> Going green? See the top 12 foods to eat organic.
>
>
http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51N1653A_______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 6
Date: Wed, 9 Apr 2008 12:39:35 -0500
From: "Douglas D Deltour"
Subject: RE: SPAM-LOW: [Histonet] Immunohistochemistry Machine
To: "'Ramona Turner'" ,
Message-ID:
Content-Type: text/plain; charset="us-ascii"
Romona,
Ventana NexES IHC. It is small and fully automated.
http://www.ventanamed.com/products/instruments/nexes_ihc.html
If you have a little more space, take a look at the BenchMark XT.
http://www.ventanamed.com/products/instruments/xt.html
Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262
Doug@ppspath.com
*****************************************************
PROFESSIONAL PATHOLOGY SERVICES, PC
NOTICE OF CONFIDENTIALITY
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law. If the
reader
of this message is not the intended recipient, you are hereby notified
that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law. If you have received this communication in
error, please notify me immediately.
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ramona
Turner
Sent: Wednesday, April 09, 2008 11:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] Immunohistochemistry Machine
I am looking for a machine to process immunohistochemistry stains. I
want
something small, that will do as much online as possible with little
hands
on attention required. Any recommendations?
Ramona
Potomac Hospital
_________________________________________________________________
Going green? See the top 12 foods to eat organic.
http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51N165
3A_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Wed, 9 Apr 2008 11:44:38 -0500
From: "Sebree Linda A."
Subject: RE: SPAM-LOW: [Histonet] Immunohistochemistry Machine
To: "Douglas D Deltour" , "Ramona Turner"
,
Message-ID:
Content-Type: text/plain; charset="US-ASCII"
Caution about the NexES IHC module; I don't believe that Ventana is
making it anymore although they may still be selling it. You wouldn't
want to get in a situation where can correct me if I'm wrong about this.
Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas
D Deltour
Sent: Wednesday, April 09, 2008 12:40 PM
To: 'Ramona Turner'; histonet@lists.utsouthwestern.edu
Subject: RE: SPAM-LOW: [Histonet] Immunohistochemistry Machine
Romona,
Ventana NexES IHC. It is small and fully automated.
http://www.ventanamed.com/products/instruments/nexes_ihc.html
If you have a little more space, take a look at the BenchMark XT.
http://www.ventanamed.com/products/instruments/xt.html
Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262
Doug@ppspath.com
*****************************************************
PROFESSIONAL PATHOLOGY SERVICES, PC
NOTICE OF CONFIDENTIALITY
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law. If the
reader
of this message is not the intended recipient, you are hereby notified
that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law. If you have received this communication in
error, please notify me immediately.
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ramona
Turner
Sent: Wednesday, April 09, 2008 11:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] Immunohistochemistry Machine
I am looking for a machine to process immunohistochemistry stains. I
want
something small, that will do as much online as possible with little
hands
on attention required. Any recommendations?
Ramona
Potomac Hospital
_________________________________________________________________
Going green? See the top 12 foods to eat organic.
http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51
N165
3A_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
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End of Histonet Digest, Vol 53, Issue 14
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