>> We just X-ray the specimens, which avoids the risk of breaking/bending and> it's easy to do all the samples at once.> ---------------------------------------------------------------------->> Message: 1> Date: Thu, 27 Mar 2008 07:57:49 -0700 (PDT)> From: Rene J Buesa > Subject: Re: [Histonet] Decal procedures-assessing endpoints> To: Greg Dobbin , Histonet@lists.utsouthwestern.edu> Message-ID: <448114.89650.qm@web65708.mail.ac4.yahoo.com>> Content-Type: text/plain; charset=iso-8859-1>> The "softness" of the tissue fragment, how it bends or compresses, will> give you the most practical way of determining the decal end point.>  Be careful with the bending to prevent breaking a piece not decalcified> yet.>  René J.>> Greg Dobbin  wrote:>  Hello Colleagues,> Can some of you share with me, your guidelines for determining> appropriate submersion times for sections of bone or other calcified> bits in RDO (Trade name) post-fixation but prior to processing> (obviously). I guess it is a subjective call by necessity!?> Thanks,> Greg>