Oil red O is a stain for lipids. It must be done on frozen sections, as processing for paraffin embedding dissolves lipids. You should use snap-frozen tissue, either fresh or formalin-fixed. I have no technical pointers (not being involved in that part of the work), but the technique is fairly common and described in many texts. The resulting stain can be a bit messy, especially with very fatty tissues. Good luck !
Jean-Martin Lapointe, DMV, MS, dACVP
1635 Lionel-Bertrand, Boisbriand
Québec, Canada J7H 1N8
tel: 450-435-9482 ext.247
Date: Thu, 27 Mar 2008 07:42:33 -0700 (PDT)
From: Jennifer Sipes
Subject: [Histonet] Oil Red Staining
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I had a investigator come to me this morning and ask if I've ever done Oil Red Staining for hepatic steatosis before. I have never heard of this. Is it easy and does someone have a protocol for it or maybe a better way of staing for hepatic steatosis if this is no longer done?
Thanks for your time gang! You guys are the best!
Jen at Hopkins
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