Hi Jennifer,
Oil red O is a stain for lipids. It must be done on frozen sections, as processing for paraffin embedding dissolves lipids. You should use snap-frozen tissue, either fresh or formalin-fixed. I have no technical pointers (not being involved in that part of the work), but the technique is fairly common and described in many texts. The resulting stain can be a bit messy, especially with very fatty tissues. Good luck !

Jean-Martin

__________________________________
Jean-Martin Lapointe, DMV, MS, dACVP
Vice-President, Pathologie
AccelLAB Inc
1635 Lionel-Bertrand, Boisbriand
Québec, Canada  J7H 1N8
tel:  450-435-9482 ext.247
fax: 450-435-4795
jm.lapointe@accellab.com
 
 


-----Original Message-----
Message: 25
Date: Thu, 27 Mar 2008 07:42:33 -0700 (PDT)
From: Jennifer Sipes 
Subject: [Histonet] Oil Red Staining
To: Histonet 
Message-ID: <329929.37149.qm@web62414.mail.re1.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Hello all!

I had a investigator come to me this morning and ask if I've ever done Oil Red Staining for hepatic steatosis before.  I have never heard of this.  Is it easy and does someone have a protocol for it or maybe a better way of staing for hepatic steatosis if this is no longer done?

Thanks for your time gang!  You guys are the best!

Jen at Hopkins


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