[Histonet] ERCC1 IHC

From:"Min-Han Tan"

Dear fellow Histonetters,

I am planning to start immunostaining FFPE lung tissue for ERCC1.

The cited antibody in the relevant New England Journal of
ERCC1 Ab-2 (Clone 8F1), Neomarkers Cat. #MS-671-R7 (7.0ml)
did not work well for us in the prediluted form, and I understand the
prediluted form has been withdrawn by the manufacturer as well.

I would like to ask as to which antibody all of you are using for ERCC1
staining (lung cancer tissue for platinum sensitivity). I understand some
clinical laboratories in the United States are using it on a routine basis.
I am thinking of obtaining the Abcam one (with the same clone 8F1, approved
for IHC - it might even be the same antibody as the Neomarkers one!)

Thank you!

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