We've done some fluorescent IHC against GFP, but they didn't work.
We believe that the GFP antigen(s) are lost during the decalcification process (92% EtOH 70% + 8% Nitric acid for 2 days) but, for example, anti-phospho-Histone3 is working fine.
Do you know any decalcification protocol that doesn't interfere with the antigenicity?
Mercč Martí Gaudes
Cap de Servei
Histology and Bioimaging
Centre de Medicina Regenerativa de Barcelona (CMR[B])
Dr. Aiguader, 88 7ena planta
Tel: +34 93 316 03 51
Fax: +34 93 224 10 83
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