AW: [Histonet] sections rolling - need ribbon!

From:"Gudrun Lang"

Hi René,
perhaps I have a mess in my brain and you can help me out. I always thought,
that paraffin with a low melting point is softer at roomtemperature. And
therefore would be stickier. So my opinion is: high melting point = harder,
ribbons are more difficult. Low melting point = softer, sections are sticky
and ribbons are easier.
You know, I am a sliding microtome user. So I could be wrong.

Gudrun Lang
Biomed. Analytikerin
Akh Linz
Krankenhausstr. 9
4020 Linz
-----Ursprüngliche Nachricht-----
[] Im Auftrag von Rene J
Gesendet: Mittwoch, 02. April 2008 21:41
An: Patty Dunlop;
Betreff: Re: [Histonet] sections rolling - need ribbon!

Check the type of paraffin, it may be of a very low melting point. Check
also the thickness you have set the microtome for, it may be too thick.
  René J.

Patty Dunlop  wrote:
  Hello Histonetters,

We just opened a new lab and I am practicing on all the equipment this week
to make sure everything is working properly before we go live.
Having a problem - I can't get ribbons to form on the microtome. They keep
rolling up on me and sometimes rise up with the block. I am using a brand
new Leica RM 2255, and have tried clearance angles of 4, 5, and 6. Am using
Accu-edge disposable blades, and am icing blocks before trying for
sections. We are doing mostly small GI biopsies, so they are small blocks.

Any suggestions would be appreciated!

Patty, HTL (ASCP)
Monterey Bay GI Consultants
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