Hi Vinnie

We routinely use the following mouse monoclonal antibodies on FFPE human
skeletal muscle:
Myosin I (slow), clone NOQ7.5.4D, Sigma M-8421; working conc. 5 ug Ig/ml,
HIER in 10 mM citrate buffer, pH 6.0, pressure cooker.
Myosin II (fast), clone MY-31, Sigma M.4276; working conc. 10 ug Ig/ml, HIER
as above.
Hope this helps.

Andi Kappeler
Insitute of Pathology, University of Bern, Switzerland 

-----Ursprüngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Della
Speranza, Vinnie
Gesendet: Donnerstag, 10. April 2008 16:45
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] antibodies to myosin for fast twitch and slow
twitchstriated muscle

Can someone recommend a source for antibodies to myosin that will
differentiate fast and slow twitch fibers? Preferably products you use and
are happy with.

Thank you,

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue Suite 309
Charleston, South Carolina 29425
Ph: (843) 792-6353
Fax: (843) 792-8974



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