After dewaxing  the slides and taking down to water
try 10 minutes in 10%NBF, rinse well before retrieval.
Pat valente
uropathllc

--- crochieresteve@aol.com wrote:

> Are there any suggestions to help keep prostate core
> biopsies on the plus slides when doing PIN-4 IHC. I
> use the Biocare de-cloaker and their BORG retrieval
> solution and Nemesis autostainer. I have been having
> a tough time with sections falling away after
> retrieval. I air dry them overnight and incubate in
> a 60 degree oven for 1 hour prior to de-wax and
> staining. What to do??
> Steve Crochiere
> Histology Supervisor
> Life Path Partners, Mercy Medical Center
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> 


Pat


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