Re: [Histonet] prostate bx falling off slide

From:Rene J Buesa

Check your processing protocol. Maybe they are treated too long in dehydrant/clearant. How do they section, brittle? If that is the case it is an indicaiton of a too prolonged processing protocol.
  René J. wrote:
  Are there any suggestions to help keep prostate core biopsies on the plus slides when doing PIN-4 IHC. I use the Biocare de-cloaker and their BORG retrieval solution and Nemesis autostainer. I have been having a tough time with sections falling away after retrieval. I air dry them overnight and incubate in a 60 degree oven for 1 hour prior to de-wax and staining. What to do??
Steve Crochiere
Histology Supervisor
Life Path Partners, Mercy Medical Center
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