Re: [Histonet] eyeball holder

From:Derek Papalegis

You should remove the eyes from Davidson's after 24 hours and place them 
into 70% alcohol until you are ready to process them. I have found this 
to be the best way to get the best mouse eye sections. Cutting mouse 
eyes can be very tedious if you need to see particular areas of the eye 
that are at different levels. I recently had a study where all my 
sections needed to show the optic nerve head. I found that there really 
was no easy way to do this since each eye was different in size and I 
couldn't find any standardization as to how deep to cut into it to show 
that particular feature. I would recommend taking some practice eyes and 
cutting those to ensure that your procedures and techniques are 
suitable. I have never used an eyeball holder and I don't think that 
they are necessary as long as you leave the optic nerve attached to the 
eye. This helps with orientation.

 I hope this helps.


Derek Papalegis HT (ASCP)
Division of Laboratory Animal Medicine
Tufts University 
136 Harrison Avenue
Boston, MA 02111
phone: 617 636-2971
fax: 617 636-8354

Ryan Dominique Salazar wrote:
> Hi Histopeeps!
>   Can you provide us a protocol for processing eyeball specimen for primates/rodents?
>   Wer'e conducting a study for opthalmology and we're starting to validate some procedures which you might help.
>   We're fixing them in davidson's fluid but we're not sure how many days?
>   Do we need an eyeball holder for embedding them?
>   Thanks a lot guys and have a blessed weekday ahead.
>   Ryan Dominique Salazar
>   Maccine Pte Ltd
> ---------------------------------
> Never miss a thing.   Make Yahoo your homepage.
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