Have you tried just air-drying the slide?  Either way, you need to make sure
the slide is air-dried before fixing with anything.

On Thu, Mar 6, 2008 at 5:57 AM, Min-Han Tan  wrote:

> Dear Histonetters,
>
> Thanks in advance for your advice.
>
> I am interested in a possible red blood cell membrane protein, and am
> trying
> to stain it on a charged slide.
>
> The cells float off after paraformaldehyde 4% fixation, but they are fine
> after methanol fixation.
>
> Unfortunately, I do not see much staining of my protein of interest with
> the
> latter fixing method.
>
> Does anyone have any experience fixing RBC smears on slides so that the
> RBCs
> do not drift off? Thanks!
>
> --
> /min-han
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