Have you tried just air-drying the slide? Either way, you need to make sure
the slide is air-dried before fixing with anything.
On Thu, Mar 6, 2008 at 5:57 AM, Min-Han Tan wrote:
> Dear Histonetters,
> Thanks in advance for your advice.
> I am interested in a possible red blood cell membrane protein, and am
> to stain it on a charged slide.
> The cells float off after paraformaldehyde 4% fixation, but they are fine
> after methanol fixation.
> Unfortunately, I do not see much staining of my protein of interest with
> latter fixing method.
> Does anyone have any experience fixing RBC smears on slides so that the
> do not drift off? Thanks!
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