Perform a Giemsa counter stain after the slides come off the stainer. The 
Giemsa will color the melanin pigment green.

Jim Burchette, HT(ASCP) QIHC
"A simple histotech from a little country hospital in North Carolina"



"Cynthia Robinson"  
Sent by: histonet-bounces@lists.utsouthwestern.edu
03/07/2008 01:34 PM

To
"histonet" 
cc

Subject
[Histonet] Melanin pigment






Has anyone successfully removed melanin pigment prior to IHC staining with 
MART-1?  Currently we use Ventana I-View DAB and I have suggested 
switching to AEC for these cases but our paths don't want to spend the 
money for a second detection system.  Any suggestions?
 
Thanks......Cindi
 
Cindi Robinson HT(ASCP)
Mercy Medical Center-Sioux City
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
(712)279-2768
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet