Re: [Histonet] Melanin pigment

From:James L Burchette

Perform a Giemsa counter stain after the slides come off the stainer. The 
Giemsa will color the melanin pigment green.

Jim Burchette, HT(ASCP) QIHC
"A simple histotech from a little country hospital in North Carolina"

"Cynthia Robinson"  
Sent by:
03/07/2008 01:34 PM


[Histonet] Melanin pigment

Has anyone successfully removed melanin pigment prior to IHC staining with 
MART-1?  Currently we use Ventana I-View DAB and I have suggested 
switching to AEC for these cases but our paths don't want to spend the 
money for a second detection system.  Any suggestions?
Cindi Robinson HT(ASCP)
Mercy Medical Center-Sioux City
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
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