Re: [Histonet] Lung processing

From:"louise renton"

Are you loading more cassettes into the new processor? are they
perhaps packed more tightly than before? - this may affect the effect
of vacuum on the "inner" cassettes in relation to those on the
just my 2c worth

On 3/13/08, Weaver, Colin  wrote:
> Hi - everyone we have just moved from routine processing in a VIP 1000
> to a Thermo-Shandon Excelsior. This has been working perfectly except
> when processing lung, especially spongy lung. When embedding after
> processing wax infiltration appears to be patchy, bubbles come out of
> the tissue when pressing the tissue into a mould, after trimming there
> are "holes" in the tissue, and sections often crumble because of lack of
> wax support. We have vacuumn on everything from first alcohol onwards.
> Anyone got any suggestions as to why this is happening? We used to use
> Thermo-Shandon Hypercenter XP's and had the same problem with lung, is
> it a Thermo thing?
> Colin
> Histology Dept
> VLA Thirsk
> Sunny Yorkshire
> Veterinary Laboratories Agency (VLA)
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Louise Renton
Bone Research Unit
University of the Witwatersrand
South Africa
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