Are you staining mouse tissue or human tissue? If mouse tissue, the vWF antibody from DAKO as well as the goat polyclonal anti-VE-Cadherin Ab from R&D work very well. If human tissue, the anti-CD31 from DAKO, clone JC/70 is excellent. 



----- Original Message -----
From: jstaruk 
Date: Wednesday, March 5, 2008 2:05 pm
Subject: [Histonet] CD31 vs. Von Willebrand Factor

> Hello all,
> 
> I have a request for a CD31 looking for angiogenesis.  I know this 
> is a very
> problematic antibody when working with paraffin sections.  Can the Van
> Willebrand antibody be substituted to obtain the same results?
> 
> Thank you
> 
> Jim
> 
> _____________________
>     Jim Staruk
> Mass Histology Service
> www.masshistology.com
> 
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> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


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