You're right that both of these antibodies will stain blood vessels.
Depending on what's going on (what disease or process is occuring), you may
find that these two antibodies don't always stain the same exact cells.
I know that sometimes there is problem using CD31 on mouse tissues... is
that what you need to do? If you're staining human tissue, I'd suggest
using CD31 (since that's what the researcher/doc asked for). If its mouse
tissue that you're dealing with, maybe someone on the list can suggest a
CD31 antibody that they know definately works on mouse tissue. Let us know
what kind of tissue and from what species you're staining, and I'm sure
you'll get more specific help.
On Wed, Mar 5, 2008 at 11:05 AM, jstaruk wrote:
> Hello all,
> I have a request for a CD31 looking for angiogenesis. I know this is a
> problematic antibody when working with paraffin sections. Can the Van
> Willebrand antibody be substituted to obtain the same results?
> Thank you
> Jim Staruk
> Mass Histology Service
> Histonet mailing list
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