RE: [Histonet] Washing out formalin fixation

From:"Rittman, Barry R"



Rene
I disagree.
Depends on what you call fixation.
If tissue is fixed for a short period of time (say 1-2 hours) then the bonds that are formed are not permanent and can be broken by washing in running tap water.
If however you fix for several hours the this becomes much more difficult due to extensive cross linking.
Barry

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, March 03, 2008 4:16 PM
To: Johnson, Teri; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Washing out formalin fixation

You cannot "unfix" a formalin fixed tissue with running water. It does not work that way.
  René J.

"Johnson, Teri"  wrote:
  Last week, a researcher here asked me what the chemical mechanism was of washing out the effects of formalin fixation on the tissues with running water. In other words, how does it work? Anybody here know?

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110


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