Use ALk Phos red instead of DAB for the staining , use of any acid attacks the quality of staining, melanin is to hard to remove from any specimen
Tere Hodges> Date: Sat, 8 Mar 2008 08:22:11 -0800> From: rjbuesa@yahoo.com> To: Robinsoc@mercyhealth.com; histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Melanin pigment> CC: > > Cynthia:> The method is simple, BUT you have to be sure that the sections are completely adhered to the slides.> Treat them with a 0.1% aq. sol. of potassium permanganate. Check under the microscope to see the melanin changed color.> Wash with abundant dist. water> Treat the sections with 0.3% aq. oxalic acid until there is no more color in the section.> Wash and procede with yout IHC protocol.> René J.> > Cynthia Robinson  wrote:> Has anyone successfully removed melanin pigment prior to IHC staining with MART-1? Currently we use Ventana I-View DAB and I have suggested switching to AEC for these cases but our paths don't want to spend the money for a second detection system. Any suggestions?> > Thanks......Cindi> > Cindi Robinson HT(ASCP)> Mercy Medical Center-Sioux City> Dunes Medical Laboratories> 350 W Anchor Dr> Dakota Dunes SD 57049> (712)279-2768> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > ---------------------------------> Looking for last minute shopping deals? Find them fast with Yahoo! Search.> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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