I have been using secondary antibodies conjugated to Qdots for more than a year now. With high success in staining, I had started switching my immunofluorescence protocols from Alexa conjugated secondaries to Qdot conjugated secondaries.
Up until recently everything worked well ... and all my slides (4%PF or 10%NBF animal tissue, processed routinely - paraffin embedded) were remaining fluorescent.
A few months passed by (as worked and studies shifted to other priorities) and this past February I picked up using Qdots again. With all new reagents (Qdot secondaries, buffer, etc), but the same protocol - I ran my immunos. However, in every case, my fluorescence was complete quenched (gone) the following day.
Is anyone who also uses Qdots experiencing this lately?
I have spoken with Invitrogen (Molecular Probes) and they have described this as a fairly recent problem and their R&D group is looking into it. However, their marketing team continues to push Qdots as brighter and longer lasting than conventional organic dyes and Alexa dyes.
Any information would be appreciated.
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