I am doing an IF double staining  on platelet markers, CD42c, PF4, CD61 and PSTPIP1 on a cytospin prepared slides. I need  help on the cytospin preparation on a clotted blood buffy coat not on a anticoagulated blood. If you can share me a protocol that you have been using will be appreciated. Thank you very much.

Reuel Cornelia, BS MT, AMT
Cellular Pathology
Texas Scottish Rite Hospital for Children
2222 Welborn Street
=0ADallas, TX 75219
Tel: 214-559-7766
fax: 214-559-7768

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