Has anyone successfully removed melanin pigment prior to IHC staining with MART-1?  Currently we use Ventana I-View DAB and I have suggested switching to AEC for these cases but our paths don't want to spend the money for a second detection system.  Any suggestions?
 
Thanks......Cindi
 
Cindi Robinson HT(ASCP)
Mercy Medical Center-Sioux City
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
(712)279-2768
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