Just soak them in xylene, but you have to be patient, usually the
coverslips take like three days to fall off alone. 
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webber, Mark
Sent: March-14-08 1:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Removing DPX coverslips can you leave in xylene
forseveral days without affecting tissue

Have done DAB and then coverslipped with DPX I have been required to
counterstain with haematoxylin. Can the coverslips be removed by soaking
in Xylene.

 

For reasons out of my control the sections may be in Xylene for several
days 

 

Firstly will this affect the tissue? 

Secondly what would I need to do to counterstain (rehydrate/dehydrate
etc??) as have never had to do this

 

Thanks 

Mark

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