Re: [Histonet] Murine perfusion for whole autoradiography

From:Roger Moretz

When I said the drip bottles were at about 6ft, I
forgot to emphasize that this was above the floor--the
mouse was in a hood, so that's about 3ft, so the
differential between the animal and the drip bottle is
is the range of 3ft.  You might lower the drip bottle
a foot to see if that works.  We did not see any
extravascular spaces in the brain that would have
indicated that pressure was too high, and I do not
recall having seen any pressure-related artifacts in
either liver, kidney or spleen (3 other organs of
interest in various studies).  I can't think of
another way to test this other than microscopically
(well, I can, but they don't seem all that attractive
or particularly feasible).

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
Ridgefield, CT
--- Jamie E Erickson 

> HI All,
>                I saw this posting of perfusion for
> mouse brain and had to 
> ask a similar question. 
> I am working on doing biodistrubtion of whole mice
> with I125 labeled 
> antibodies. The question I have is I want to perfuse
> out all the 
> radioactive blood from my mice before sectioning the
> whole mouse  so that 
> I can look to see where the binding might be and
> have little radioactivity 
> due to blood content.  If the pressure in the
> perfusion is to high this 
> may push my radioactive antibody by pressure my 
> into an area that is not 
> where it would have gone in-vivo without perfusion
> giving me a false 
> positive location.
>       So is there a way to mimic physiological
> pressure in a mouse so that 
> this would not be an issue. How high would you hang
> the perfusion bag for 
> a mouse???
> Any thoughts???
> _______________________________
> Jamie Erickson
> Sr. Research Associate 
> Department: DSMP
> Abbott Bioresearch Center
> 100 Research Drive
> Worcester, MA 01605-4341
> 508-688-3134
> FAX: 508-793-4895
> e-mail:
> _______________________________________________
> Histonet mailing list

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