Re: [Histonet] CD138 (Clone B-B4)

From:"Andi Kappeler"

Dear May

I'm sorry, we did not try 5F7 - I had a hint from a colleague that MI15 
would probably work well for our purposes, so we didn't  spend more time and 
money to compare several possible clones (our finance people are grateful 
for that ...)
Andi Kappeler

----- Original Message ----- 
From: "May Wei" 
To: "Andi Kappeler" 
Sent: Tuesday, March 20, 2007 7:02 PM
Subject: RE: [Histonet] CD138 (Clone B-B4)

Dear Andi,

Did you try clone 5F7? If so,  how is it compared with MI15?

Thank you very much!
May Wei
BioGenex Laboratories

-----Original Message-----
[] On Behalf Of Andi
Sent: Monday, March 19, 2007 11:18 PM
To: Luck, Greg D.; Histonet
Subject: Re: [Histonet] CD138 (Clone B-B4)

Hi Greg
we were confronted with the same problem and have switched now to clone
MI15, which gives us comparable results (on human FFPE tissue, mainly
bone marrow). MI15 is from Dako, CatNo M7228. We use it at 1.8 ug Ig/ml,
HIER in citrate, pH 6.0, pressure cooker. Hope this helps.
Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

----- Original Message -----
From: "Luck, Greg D." 
Sent: Monday, March 19, 2007 10:16 PM
Subject: [Histonet] CD138 (Clone B-B4)

Hello all,
I'm looking for a source the the Ab. and clone in my subject line.  Our
previous vendor (Serotec) informed us when we attempted to reorder that
it was no longer available from them.  Thanks, Greg
Greg Luck, B.S., HT(ASCP)
Anatomic Pathology Supervisor
Deaconess Med Cntr
800 W. 5th Ave
Spokane, WA 99204
Offc 509.473.7077
Fax 509.473.7133 

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