Since spleen is so hemorrhagic I have found once the block is trimmed then prolonged soaking on a slushy, ice water bath prior to sectioning is a huge help. If that is not enough, add some ammonium hydroxide to a separate container and fill with crushed ice and add your trimmed block. Cover to help contain the fumes and this works wonders.
From: firstname.lastname@example.org on behalf of mesruh turkekul
Sent: Sun 3/18/2007 4:44 PM
Subject: [Histonet] sectioning spleen
I have tried manual paraffin embedding and embedding of spleen in the
But I have noticed it is not easy to section. The tissue is so dry and
crunchy. I really need to blow hard onto the block while sectioning to
get nice sections. Do you have any tips in terms of sectioning. Or
maybe fixation, processing?
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