[Histonet] Ig kappa with Benchmark XT

From:Malam Jacqueline

Has anyone out there got a good protocol for demonstrating Ig kappa and
lambda by IHC on the Ventana Benchmark XT using Dako's antisera codes A1091
and A 1093 and what is the dilution? I will willingly swap any of my
protocols in exchange. The UKNEQAS-ICC do not like the ISH protocol we use
(ISH protease 3 for 32 minutes) plus we are consistently getting needle-like
deposition of the iView blue chromogen despite excluding water from our
dehydrating reagents. I will be trying their ISH protease 2 shortly with the
ISH protocol so that may solve my problem but any info in the meantime would
be  most welcome.


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