[Histonet] Background

From:Pablo =?iso-8859-1?Q?S=E1nchez?= Quinteiro

Dear Histonetters,

I am doing OMP immunohistochemistry in paraffin-embedded sections of sheep
olfactory mucosa.

In my positive control -mouse olfactory tissue- there is not any
background, but in he sheep sections I get lot of unspecific labelling
(vessels, connective tissue, respiratory mucosae).

Being my primary antibody raised in goat I guess that there is an undesired
cross reaction with the sheep tissue.

Could I improve my blocking? I do half an hour in 2% BSA and 5% Horse
normal serum.

Should I use Goat normal serum?

Thanks in advance for your input.

Pablo Sanchez-Quinteiro
Lugo (Spain)

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