Dear Histonetters,
I am doing OMP immunohistochemistry in paraffin-embedded sections of sheep
olfactory mucosa.
In my positive control -mouse olfactory tissue- there is not any
background, but in he sheep sections I get lot of unspecific labelling
(vessels, connective tissue, respiratory mucosae).
Being my primary antibody raised in goat I guess that there is an undesired
cross reaction with the sheep tissue.
Could I improve my blocking? I do half an hour in 2% BSA and 5% Horse
normal serum.
Should I use Goat normal serum?
Thanks in advance for your input.
Pablo Sanchez-Quinteiro
Lugo (Spain)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
|