AW: [Histonet] DAB & Masson's compatability

From:"Gudrun Lang"

The trichrome-dyesolutions usually have a ph from 1-3. I think, that is
strongly acid.
My tipp is, to try it on a control slide and see what happens. Or just rinse
the ready stained ihc-slide in an acid solution.
If you don't mind, that the trichrome results aren't very brilliant, you can
ommit the bouin-step.

Gudrun Lang
Biomed. Analytikerin
Akh Linz
Krankenhausstr. 9
4020 Linz

-----Ursprüngliche Nachricht-----
[] Im Auftrag von Geoff
Gesendet: Freitag, 30. März 2007 22:26
An: Walters, Katherine S
Betreff: Re: [Histonet] DAB & Masson's compatability

I would think it would be fine as long as you avoid strong acids. Try it 
on a spare slide before doing the important stuff. Also, intensifying 
the DAB with nickel, nickel+cobalt, osmium vapors, silver or a 
commercial product like Vector's Intense would be worth a try.


Walters, Katherine S wrote:

>Question-Will a slide stained with DAB hold up to a Masson's trichrome
>stain?  I am worried about losing the DAB precipitate.  Specifically, I
>want a stain that will demonstrate fibrosis and a cytokine antibody on
>the same slide.  Does anyone have any ideas about how to do this?
>Histonet mailing list

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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