Kris:
IHC tends for some markers to work better on frozens due to the lack of  
crosslinked proteins from fixation. It has been my experience when doing IHC to  
usually lessen incubation times to eliminate high backgrounds and overstaining, 
 or to use very high titers, but this means you need to do studies and 
titrations  to determine optimal conditions for frozen section IHC before 
standardizing any  protocol.
 
 
Dana Dittus
Biosciences Product Manager
Polysciences,Inc.
 



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