Even in research, our positive control always contains fungus. For kidney
tissue, especially renal biopsies, we do the PAS differently than for
fungus anyway, plus the sections were always cut at 2 um in order to see
the thin basement membrane staining.
We do not use Periodic acid as the oxidizer for fungus staining, we prefer
using chromic acid, much stronger in order to get better positive staining
of a fungus. As an aside, Frieda Carson and Jerry Fredenburgh published in
Journal of Histotechnology, the pitfalls of getting false negatives with
PAS for fungus staining.
a positive control for PAS should contain fungus. At 07:25 AM 3/7/2007,
>Is it acceptable to use kidney tissue as a control for PAS stain when
>looking for fungus? We have been having a debate on this issue and would
>like to know what you all think.
>Meera Bansal MD
>Mercy Medical Center
>Rockville Centre, NY
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-4303 (FAX)
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