Remove the other organs first (clamp off the blood supply) then perfuse.
Geoff
scoop@mail.nih.gov wrote:
> Dear Histonetters,
>
> I have been using fixed mouse femur to do mouse bone marrows. I get
> much better fixation and preservation of bone marrow if I perfuse the
> mouse than if I immersion fix the femurs. However, I prefer not to
> perfusion fix the mouse because perfusion fixation prevents me from
> collecting other tissues unfixed. Is there a way to improve the
> fixation of the mouse femurs when I immersion fix? I'm guessing that
> the problem is I'm not getting good enough penetration - I suppose I
> could poke a few holes in the femurs?
>
> Thanks in advance for your help,
> Sharon
>
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>
--
--
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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