Re: [Histonet] GAG Stain

From:Geoff McAuliffe

Alcian Blue at pH 2.5 or 1.0, precceded by digestion with specific 
enzymes, will work for formalin-fixed (better is formalin-alcohol-acetic 
acid) tissues.
There is also the "critical electrolyte concentration" methods with 
Alcian Blue.
Some lectin methods (see Vector Labs website) will demo specific amino 
sugars of the GAG.

Geoff wrote:

>Hello All,
>I'm trying to find a staining protocol for Gycosaminoglycans (GAGs).   Does 
>anyone have a protocol that will work on fixed sheep tissues?
>Albert C.  Grobe, PhD
>International Heart Institute of Montana Foundation
>Tissue  Engineering Lab, Saint Patrick  Hospital

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