A blocking agent can be other than a normal serum block and commonly sold
by the various vendors but it pays to try these out before using them all
We use a normal serum block and always match the normal serum block to the
HOST of the secondary. Then I would be using Donkey in your case. I have
seen people use swine serum too but we just follow match to the host rule
and very few problems with our murine work.
At 09:41 AM 3/8/2007, you wrote:
>We have been hotly debating this subject for over a week in our lab.
>How do you choose the blocking reagent for immunohistochemistry?
>If you are using a secondary made in a donkey can you just use any hoofed
>animal's serum to block??
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-4303 (FAX)
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