RE: [Histonet] goldfish processing

From:"Monfils, Paul"

Given the information you provided, I would say cut the sections in whatever plane will provide the greatest surface area to view (presumably sagital). While I don't have much experience sectioning fish gills, I assume they will be rather curved when excised. You might want to hold them flat during processing, so it will be easier to get a complete section.  For this purpose I use cassette lids from which I have removed the outer rim. This gives me a flat, rigid, solvent-resistant "screen" that just fits inside a cassette. When I have a curved, flexible tissue that I want to process flat, I place the tissue in the cassette, drop one of these modified lids on top of it (or even two of them if the tissue is very thin), then close the cassette lid as usual. The tissue is pressed lightly between the "inner lid" and the cassette bottom, thereby remaining flat during processing.

> ----------
> From: on behalf of Earle, Elizabeth
> Sent: 	Wednesday, March 7, 2007 6:58 AM
> To:
> Subject: 	[Histonet] goldfish processing
> I have some goldfish which have been placed intact into formalin. They
> were exposed to different contaminants in water before being sacrificed.
> What is the best way to demonstrate the gills? Should I do cross
> sections or sagital sections? Thanks for any help; if someone can point
> me to a reference that would be great too.
> PS this is for a science fair project, favor for a friend.
> Thanks
> EE
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