RE: [Histonet] fungus control for PASfungus

From:"Tony Henwood"

Acceptable but not optimal.

Fungus tends to stain differently to basement membranes with some
variability depending on the fungus type and its age (dead fungus tends
to stain poorly - need a Chromic acid - Methenamine Silver or a Chromic
acid - Schiffs reaction to better show them).

It is easy to make your own Fungi control. Fix and process some slices
of mouldy bread or fruit.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

>	Bandaged Bear Day - Friday 30th March 2007
>	For more information and to order merchandise visit
www.bandagedbearday.com.au
>
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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bansal,
Meera
Sent: Thursday, 8 March 2007 1:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] fungus control for PASfungus


Is it acceptable to use kidney tissue as a control for PAS stain when
looking for fungus? We have been having a debate on this issue and would
like to know what you all think. Meera Bansal MD Mercy Medical Center
Rockville Centre, NY


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