RE: [Histonet] Choosing a blocking reagent

From:"Liz Chlipala"


We use a serum free protein block, many vendors have them we get ours from
Dako.  It makes things a bit easier for us, we no longer need to worry about
which species or detection system we are using the serum free protein block
will work on all of them.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
[] On Behalf Of Jennifer
Sent: Thursday, March 08, 2007 9:42 AM
Subject: [Histonet] Choosing a blocking reagent

We have been hotly debating this subject for over a week in our lab.

How do you choose the blocking reagent for immunohistochemistry?

If you are using a secondary made in a donkey can you just use any hoofed
animal's serum to block??


Jennifer Harvey

Center for Stem Cell Biology
Department of Cell and Dev Biology
Vanderbilt University
831 Light Hall
2215 Garland Ave.
Nashville, TN  37232-0225
Phone: 615-322-4378
Fax: 615-343-2173

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