RE: [Histonet] Chatter in GI Biopsies

From:"Tony Henwood"

A few thoughts,

Check the microtome - not loose?, is the knife angle right?
When the blocks are cooling prior to sectioning is the ice too cold?
(This occurred in my lab when we were freezing our ice trays in a -60oC
Is the trimming (or facing) too vigorous? Polish the block before
sectioning (take a few sections at 4-5um at normal sectioning speed and


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

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-----Original Message-----
[] On Behalf Of
Sent: Friday, 9 March 2007 5:35 AM
Subject: [Histonet] Chatter in GI Biopsies

Dear All,

We have been noticing an increase in chatter for our GI biopsies over
the last month or so.  We use both microwave (Milestone) and
conventional (VIPs) processors.  We were using the microwave mainly for
small biopsies with very good results until the increase in chatter
occured.  We switched to the conventional processors and got about the
same results.  The temps look fine as well as the solutions.   Does
anyone out there have an idea on this problem.  At this point in time, I
am open to just about all suggestions. I can be emailed at my work:
mailto:elonergan@convergedx.     Thanks.

Eileen Lonergan HT(ASCP)
ConVerge Diagnostic Services
Peabody MA 01960

Eileen Lonergan
(207) 749-9617  cell
(207) 324-6468  home

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