The control slides for Fouchet should  be cut only when you need them they 
deteriorate once cut.
The stain should turn the bile a bluish green as opposed to the intrinsic 
 yellowish green of the bile.


Rosalba Zumbo
Supervisor Histology Dept
Department of Forensic Medicine
42-50 Parramatta Rd
Glebe NSW 2037
Australia
PH: 61 2 85847842
FAX: 61 2 95664573
zumbor@email.cs.nsw.gov.au


-----Original Message-----
From:	W. Ozanne [SMTP:wayneozanne@sympatico.ca]
Sent:	Wednesday, 14 March 2007 9:14 AM
To:	histonet@lists.utsouthwestern.edu
Subject:	[Histonet] A Good Bile Control for Fouchet's Procedure

To the experts,

I currently have no good positive control for the Fouchet's SOP  for
detecting bile.  We are currently using old, pre-cut positive control 
slides
that I am sure at some point demonstrated a strong positive staining
reaction.  We are currently seeing positive green staining, however it is
very light.  I am certain this is from the old, precut slides.

Any recommendations?  Has anyone stained gall bladder and is it positive?

Thanks for your ideas and suggestions.

Wayne Ozanne, Chief Technologist & Laboratory Safety Officer
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