Before I go into detail, I am aware of the most recent exchange re: avidin biotin complex (ABC) kits from Laura Harris and Gayle Callis (Histonet Digest, Vol. 40, Issue 17) and that Vector's mouse on mouse ABC kit is problematic while DAKO's ARK kit and Biocares MM kit seem to decent with regards to background. Are these results confined to only frozen sections? My plan was to do immunohistochemisty (IHC) of chondroitinase ABC-treated and untreated paraffin-embedded vocal fold tissues. Any opinions for this neophyte?
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