[Histonet] ovarian tissue coming off slides

From:Heather A Leba

After a month of trying to troubleshoot this issue can anyone offer some suggestions? I'm mounting fish ovarian tissue and ooctyes and have repeadedly had my sections fall off during the staining process (H & E) during the second xylene step and also during the ETOH. I've tried using tissue from different specimens, replacing my solutions during the dehydration process, changing the staining solutions, using poly-L-lysine coated slides, putting them on a slide warmer, letting them air dry overnight, and now tried charged slides (but have not stained these yet). I'm not sure what else to try and am at my wits end. Please help!

Heather Leba

UH Manoa, Zoology Dept.

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