Dear Histonetters,
I have been using fixed mouse femur to do mouse bone marrows. I get
much better fixation and preservation of bone marrow if I perfuse the
mouse than if I immersion fix the femurs. However, I prefer not to
perfusion fix the mouse because perfusion fixation prevents me from
collecting other tissues unfixed. Is there a way to improve the
fixation of the mouse femurs when I immersion fix? I'm guessing that
the problem is I'm not getting good enough penetration - I suppose I
could poke a few holes in the femurs?
Thanks in advance for your help,
Sharon
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