[Histonet] does anyone know how to get better immersion fixation of mouse femur?


Dear Histonetters,

I have been using fixed mouse femur to do mouse bone marrows.  I get 
much better fixation and preservation of bone marrow if I perfuse the 
mouse than if I immersion fix the femurs.  However, I prefer not to 
perfusion fix the mouse because perfusion fixation prevents me from 
collecting other tissues unfixed.  Is there a way to improve the 
fixation of the mouse femurs when I immersion fix?  I'm guessing that 
the problem is I'm not getting good enough penetration - I suppose I 
could poke a few holes in the femurs?

Thanks in advance for your help,

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