Wayne Ozanne (where in Canada?) writes:
>>I currently have no good positive control for the Fouchet's SOP for
detecting bile. We are currently using old, pre-cut positive control
slides that I am sure at some point demonstrated a strong positive
staining reaction. We are currently seeing positive green staining,
however it is very light. I am certain this is from the old, precut
slides. - Has anyone stained gall bladder and is it positive?<<
I've never known anyone to actually do a bile stain, because bile is
usually obvious in the H & E. Oxidizing agents oxidize yellow-brown
bilirubin to green biliverdin. Hall's technique (1960) uses Fouchet's
(pronounced foo-SHAY) reagent (1917), a fresh mixture of trichloracetic
acid and ferric chloride, as the oxidizing agent. (All this from
Bancroft & Stevens).
Lee Luna in the AFIP manual has a complex method for meconium in
placentas that may be worth looking at. The issue is rarely addressed,
but I think that meconium has the same staining reactions as ordinary
bilirubin, so that you might be able to use a block of rolled fetal
membranes with meconium macrophages (very common) as a control. (If you
try this, please let us all know the results.)
An occasional section of gallbladder will have some stainable bile, but
I'd prefer liver - probably from an autopsy - with bile stasis. Could
you locate such a case in your files?
Bob Richmond
Samurai Pathologist
Knoxville TN
________________________________________________________________________
AOL now offers free email to everyone. Find out more about what's free
from AOL at AOL.com.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
|